Effects of low dose oxymatrine on mouse lymphocyte proliferation stimulated by Con A |
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Authors: | Li Luo-si Wu Bin Li Jian-guo Xie Hong-fu Zhang Yang-de Cong Ling and Shi Jun |
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Affiliation: | (1) Institution of Biomedical Engineering, Central South University, 410078 Changsha, China;(2) Department of Dermatology, Xiangya Hospital, Central South University, 410008 Changsha, China;(3) Department of Dermatology, the First Affiliated Hospital, Jinan University, 510630 Guangzhou, China |
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Abstract: | Objective To investigate the effects of low dose Oxymatrine (OMT) on mouse lymphocyte proliferation stimulated by Con A making use
of fluorescence dyestuff (CFDA-SE). Methods CFDA-SE staining and flow cytometry were used to detect the fluorescence intensity of lymphocytes after stimulated by polyclonal
stimulators Con A and OMT. Then, related software was used to analyze the effects of OMT on mouse lymphocyte proliferation.
Results After cultured for 48 h, CFSE fluorescence could be detected by cytometer, filial generation peaks did not appear in control
group, which indicated that lymphocytes did not proliferate. Three peaks were obviously detected in Con A group which indicated
that Lymphocytes divided after 48 h stimulated by Con A compared with the halving of the fluorescence intensity of control
group. In groups with Con A and OMT treated. Primary generation peaks are all lower while filial generation peaks are significantly
higher than groups with Con A treated only. This indicated OMT obviously promote lymphocyte proliferation. After cultured
for 72 h, the fluorescence intensity changes between all groups are consistent with those of cultured for 48 h. Analyzed with
CELLQuest software, it is shown that OMT could promote lymphocyte proliferation in 16, 8, 4 and 2 μg/mL respectively. Conclusions 1)CFDA-SE dyeing and flow cytometer were both reliable tools to analyse lymphocyte proliferation; 2) lower dosage of OMT
could promote the proliferation of lymphocyte as a immunopotentiator.
Correspondence |
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Keywords: | CFDA-SE OMT lymphocyte proliferation |
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