| 双波长分光光度法测定维生素B_(12)在糖液中的稳定性 |
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| 引用本文： | 曾建成,于淑娟,张建俊.双波长分光光度法测定维生素B_(12)在糖液中的稳定性[J].现代食品科技,2009,25(1). |
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| 作者姓名： | 曾建成 于淑娟 张建俊 |
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| 作者单位： | 华南理工大学轻工与食品学院,广东,广州,510640 |
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| 摘 要： | 采用双波长分光光度法,排除羟钴胺素干扰,测定维生素B12在糖液中的含量变化,测定主波长为356.0nm,参比波长为344.7 nm,平均回收率为101.71%,RSD为4.41%(n=6);结果表明,加糖后维生素B12在日光灯照射存放3 d过程中有不同程度的降解,其中低聚半乳糖、低聚异麦芽糖和麦芽糖变化最为明显,而在避光存放过程中均无明显降解;随着蔗糖浓度增加,维生素B12降解程度呈现先升后降的趋势.
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| 关 键 词： | 双波长分光光度法 稳定性 维生素B12 羟钴胺素 糖 |
Stability of Vitamin B_(12) in Sugar Solutions by Double-wavelength Spectrophotometry |
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| ZANG Jian-cheng,YU Shu-juan,ZHANG Jian-jun.Stability of Vitamin B_(12) in Sugar Solutions by Double-wavelength Spectrophotometry[J].Modern Food Science & Technology,2009,25(1). |
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| Authors: | ZANG Jian-cheng YU Shu-juan ZHANG Jian-jun |
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| Affiliation: | College of Light Industry and Food Sciences;South China University of Technology;Guangzhou 510640 |
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| Abstract: | In this paper,double-wavelength spectrophotometry was used to determine the Vitamin B12 in sugar solutions with detective wavelength and references of 356.0nm and 344.7nm,respectively.The recovery and RSD were of 101.71%,and 4.41%(n=6),respectively.Exposed to daylight for 3 days,Vitamin B12 degraded obviously in presence of seven kinds of sugar,especially oligo-galactose,oligoisomaltose and maltose,while stored protecting from light,it showed no obvious changes.The degradation of Vitamin B12 increased first... |
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| Keywords: | double-wavelength spectrophotometry stability vitamin B12 hydroxocobalamin sugar |
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