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Proton nuclear magnetic resonance rapid and structure-specific determination ofω-3 polyunsaturated fatty acids in fish lipids |
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| Authors: | Raffaele Sacchi Isabel Medina Santiago P Aubourg Francesco Addeo Livio Paolillo |
| Affiliation: | (1) Istituto di Industrie Agrarie, Università di Napoli Federico II, Parco Gussone, 80055 Portici, Italy;(2) Present address: Instituto de Investigaciones Marinas del CSIC, Eduardo Cabello, 6, 36208 Vigo, Spain;(3) Present address: Dipartimento di Chimica, Università di Napoli Federico II, via Mezzocannone 4, 80134 Napoli, Italy |
| Abstract: | Based on proton nuclear magnetic resonance (1H-NMR) spectroscopy, a rapid and structure-specific method for the determination of ω-3 polyunsaturated fatty acids (PUFAs) in fish lipids is presented. The different chemical shift observed for the methyl resonance of ω-3 PUFAs (δ=0.95 ppm) with respect to the methyl resonance of all other fatty acids (δ=0.86 ppm) has provided the possibility of proposing a new and rapid method for the determination of ω-3 PUFA content. Twenty-four fish lipid samples (raw, cooked and canned albacore tuna) produced results that showed good agreement between1H-NMR analysis and gas chromatographic determination. Raw and cooked samples showed significantly higher levels of ω-3 PUFA than canned tuna. |
| Keywords: | 1D-TOCSY CG 1H-NMR ω -3 PUFA determination fresh cooked and canned tuna fish oils Thunnus alalunga lipids |
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