| 高效液相色谱测定保健食品中的褪黑素 |
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| 引用本文： | 吴红京,唐根源. 高效液相色谱测定保健食品中的褪黑素[J]. 福建分析测试, 1999, 0(2) |
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| 作者姓名： | 吴红京 唐根源 |
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| 作者单位： | 中国科学院福建物质结构研究所 福州350002 |
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| 摘 要： | 用HPLC测定具有改善睡眠等特定功能的保健食品“梦通宁”中的褪黑素,并结合紫外光谱来鉴定样品中的褪黑素。以Novapak C_(18)为分离柱,甲醇:0.05mol/L Na_2HPO_4 (30:70)作流动相,检测波长为230nm,用外标法进行定量分析,方法回收率达98.11％,检出限为0.5mg/L。
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| 关 键 词： | 高效液相色谱 褪黑素 保健食品 |
The Determination of Melatonin in Health-Caring Food by High Performance Liquid Chromatography |
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| Wu Hongjing and Tang Genyuan. The Determination of Melatonin in Health-Caring Food by High Performance Liquid Chromatography[J]. Fujian Anal Ysis & Testing, 1999, 0(2) |
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| Authors: | Wu Hongjing and Tang Genyuan |
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| Abstract: | "Meng Tong Ning" is a health-caring food for specific purpose. This parper de- scribes a method of high performance liquid chromatography for quantiating mclatonin in the products. The mclatonin in product was extracted with methanol and separated with Novapak C18 (4. 6mm i. d.×150mm)and methanol-0.05mol/L Na_2HPO_4(pH3)=30: 70 (v/v) mobile plase with detection at UV spectrum. Regression coefficient of correlation of calibration curves in the range of 1-5mg/L was 0.9999, the detection limit was 0.5mg/L. The method is simple, sensitive and suitable for routine analysis. |
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| Keywords: | high liquid performance melatonin heath-caring food |
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