食品中单核细胞增生性李斯特氏菌PCR快速检测方法

为建立食品中单核细胞增生性李斯特氏菌 (单增李斯特氏菌 )的快速、敏感、特异的PCR检测方法 ,选取hlyA基因作为靶序列设计一对引物 ,用该引物对 6 3株从国内食品中分离的李斯特氏菌 (进行传统方法验证 )和 2 0株非李斯特氏菌进行PCR扩增 ,并用此方法对人工污染食品进行检测 ,扩增片段表现出极好的单增李斯特氏菌种特异性 ,人工污染的生肉、冷冻虾仁、卷心菜的检出限为 39cfu g ,为食品中单增李斯特氏菌的快速、敏感并且特异的检测方法。

Development of a rapid,sensitive and specific polymerase chain reaction (PCR) method for detection of Listeria monocytogenes (LM) in foods

Objective: To establish a rapid, sensitive and specific polymerase chain reaction(PCR) method for detection of Listeria monocytogenes (LM) in foods. Methods: A pair of oligonucleotide primers were designed with hlyA gene as target sequence. Using the designed primers, sixty-three strains of LM isolated from different foods in different areas of chain, 3 strains of Listeria innocua and 20 strains of other bacteria were amplified by the PCR method, and the method was used to detect LM seeded onto fresh meat, frozen shrimp, and cabbage. Results: Amplified fragment showed excellent features of LM. Limit of detection for fresh meat, frozen shrimp and cabbage was 10 cfu/g. Conclusions: A rapid, sensitive and specific PCR method was established for detection of LM in food.