Comparison of sequentially combined carbon with sole carbon in denitrification and biological phosphorus removal.

The sequentially combined carbon (SCC) of methanol and acetic acid was used for the biological nutrient removal (BNR). Its BNR performance was compared with methanol or acetic acid as a sole carbon substrate. Compared to the sole carbon substrate, the use of SCC demonstrated the highest overall TIN removal of 98.3% at a COD ratio of 30 mg COD/l of methanol/50 mg CDO/l of acetic acid. Furthermore, denitrification was more enhanced when methanol was used as one of the SCC, rather than as a sole carbon source. Complete phosphorus removal was accomplished with a non-detectable o-P concentration when SCC was added. This research also showed that aerobic denitrifiers appear to prefer acetic acid to methanol, and the amount of poly-beta-hydroxybutyrate (PHB) stored by P accumulating organisms (PAOs) using acetic acid in the anoxic zone could be another important factor in improving the aerobic denitrification. The SCC was a very favorable carbon source for the aerobic denitrification since acetic acid was utilized more efficiently for P-release in accordance with increase of PHB stored in the cell of PAOs by removing nitrogen first using methanol.