复合溶剂低温结晶法富集鱼油中的棕榈油酸

为探究鱼油来源单不饱和脂肪酸的富集工艺，本研究以鳀鱼油为原料，采用复合溶剂-低温结晶法提纯POA。与单一溶剂相比，复合溶剂效果更佳。以POA含量及得率为指标，采用单因素实验对单一/复合溶剂种类、复合溶剂体积比、油溶比、结晶温度、结晶时间进行优化。确定最佳工艺条件为丙酮-乙腈体积比为1∶10，鱼油与溶剂质量比为1∶6，结晶温度为-60℃，结晶时间为6 h，得到的混合脂肪酸中POA含量为（29.75±0.53）%。进一步进行放大试验，将得率提升至50%左右，总不饱和脂肪酸含量达79%以上。本研究为ω-7单不饱和脂肪酸的富集工艺提供了参考。

Enrichment of Palmitoleic Acid in Fish Oil by Compound Solvent Low Temperature Crystallization

To explore the enrichment process of monounsaturated fatty acids from fish oil， this study used anchovy oil as raw material and purified POA using a composite solvent low-temperature crystallization method. Compared to a single solvent， composite solvents exhibited superior performance. In order to optimize the process， single-factor experiments were conducted， considering the type and volume ratio of the solvents， massratio of fish oil to solvent， crystallization temperature， and crystallization time， with POA content and yield as indicators. The optimal process conditions were determined as a volume ratio of acetone to acetonitrile of 1∶10， a mass ratio of fish oil to solvent of 1∶6， a crystallization temperature of -60 ℃， a crystallization time of 6 h， and the POA content was analyzed as (29.75±0.53)% in the obtained mixed fatty acids. Further scaling up experiments were conducted to increase the yield to around 50%， with a total unsaturated fatty acid content of over 79%. This serves as a valuable reference for the enrichment process of ω-7 monounsaturated fatty acids.