Photoautotrophic cultivation of the green alga Chlamydomonas reinhardtii as a method for carbon dioxide fixation and α-linolenic acid production |
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| Authors: | Masamichi Akimoto Hidenao Yamada Kazuhisa Ohtaguchi Kozo Koide |
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| Affiliation: | (1) Department of Chemical Engineering, Tokyo Institute of Technology, 152 Tokyo, Japan;(2) Present address: Department of Applied Chemistry and Biotechnology, Niigata Institute of Technology, 1719 Fujihashi, Kashiwazaki, 945-11 Niigata, Japan |
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| Abstract: | Focusing on CO2 fixation and α-linolenic acid (ALA) production, photoautotrophic cultivation of the green alga Chlamydomonas reinhardtii was investigated by using a culture medium of pH 6.8 under a 5 vol% CO2-enriched atmosphere. The optimum cultivation temperature and light intensity for growth were 25°C and 12 klux, respectively. The cellular ALA content nearly doubled to 11.9 mg/g of dry cells when the concentration of culture medium was doubled. Simulation of chemostat cultivation showed that the rate of CO2 fixation and ALA productivity per unit volume of culture medium could reach 1.01 kg CO2/(m3 · d) and 7.46 g ALA/(m3 · d), respectively, at a cell concentration of 0.57 kg cells/m3. |
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| Keywords: | Alga α -linolenic acid anti-tumor agent carbon dioxide Chlamydomonas reinhardtii unsaturated fatty acid |
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