| 大肠杆菌E881产苯丙氨酸转氨酶培养基的优化 |
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| 引用本文： | 刘夏忠. 大肠杆菌E881产苯丙氨酸转氨酶培养基的优化[J]. 食品科学, 2002, 23(7): 65-67 |
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| 作者姓名： | 刘夏忠 |
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| 作者单位： | 清华紫光(集团)总公司 |
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| 摘 要： | 通过正交试验,确定了大肠杆菌E881产酶的最佳培养基为:葡萄糖(1.5%)酵母膏(1%)KH2PO4(0.3%)(NH4)2SO4(0.2%)。以此培养基培养出来的游离细胞为酶源,在底物浓度为0.2mol/L时进行转氨反应,反应6h,底物摩尔转化率达到90%以上。
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| 关 键 词： | 大肠杆菌 转氨反应 L-苯丙氨酸 |
Optimization of Medium for Production of L - Phenylalanine Transaminase of Escherichia coli 881 |
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| Liu Xiazhong.. Optimization of Medium for Production of L - Phenylalanine Transaminase of Escherichia coli 881[J]. Food Science, 2002, 23(7): 65-67 |
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| Authors: | Liu Xiazhong. |
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| Affiliation: | Liu Xiazhong. |
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| Abstract: | The medium for culture and productio n of L -Phenylalanine Transaminase o f Escherichia coli 881was optimized by orthogonal experiment s.The experiment showed that the opt imal medium ingredients were:gluco se(1.5%),yeast extract(1%),KH 2 PO 4 (0.3%)and(NH 4 ) 2 SO 4 (0.2%).With the free cell as enzyme source,the trans -ratio of phenylpyruvic acid was above 90%while substr ate concentration was 0.2mol /L and transaminase reaction time 6hours. |
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| Keywords: | Escherichia coli Transaminase reaction L -phenylala nine |
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