| 牛奶样品中志贺氏菌的快速PCR检测技术研究 |
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| 引用本文： | 胡建华,李洁莉,马兆飞,陆玲. 牛奶样品中志贺氏菌的快速PCR检测技术研究[J]. 食品科学, 2007, 28(8): 433-437 |
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| 作者姓名： | 胡建华 李洁莉 马兆飞 陆玲 |
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| 作者单位： | 江苏省产品质量监督检验研究院； 南京师范大学生命科学学院微生物研究所； 南京师范大学生命科学学院微生物研究所 江苏南京210029； 江苏南京210029； 江苏南京210046； |
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| 基金项目： | 国家质检总局质量技术监督科技基金 |
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| 摘 要： | 建立牛奶中快速检测志贺氏菌的有效方法。根据GenBank公布的志贺氏菌ipaH基因的保守序列设计特异性引物,筛选合适的DNA模板制备方法,采用快速常规PCR和定量实时PCR结合,对培养液中及牛奶阳性样品中的志贺氏菌进行检测,检测敏感度可达到2CFU/ml,检出时间小于20h。新建的PCR方法具有特异性好,灵敏度高等特点,适用于快速、准确检测牛奶中志贺氏菌的需要。
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| 关 键 词： | PCR 志贺氏菌 ipaH 牛奶 Real-timePCR |
| 文章编号： | 1002-6630（2007）08-0433-05 |
| 修稿时间： | 2007-05-25 |
Detection of Shigella in Milk by Polymerase Chain Reaction (PCR) and Real-time PCR |
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| HU Jian-hua,LI Jie-li,MA Zhao-fei,LU Ling. Detection of Shigella in Milk by Polymerase Chain Reaction (PCR) and Real-time PCR[J]. Food Science, 2007, 28(8): 433-437 |
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| Authors: | HU Jian-hua LI Jie-li MA Zhao-fei LU Ling |
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| Affiliation: | 1.Jiangsu Provincial Supervising and Testing Research Institute for Products Quality, Nanjing 210029, China;2.College of Life Science,Nanjing Normal University, Nanjing 210046, China |
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| Abstract: | To establish a rapid detection technique for Shigella in the milk.One set of specific primers were designed,according to the conserved area of ipaH gene published on GenBank,to detect Shigella by using a suitable template extraction method for the polymerase chain reaction (PCR) and real-time PCR.The sensitivity achieved was as high as to the 2CFU/ml and the detection time was less than 20 h. The new established method will be used to detect the Shigella in the milk rapidly and accurately. |
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| Keywords: | PCR ipaH Real-time PCR |
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