响应面法优化普鲁兰多糖发酵培养基

通过对出芽短梗霉生长的培养基进行优化，以提高普鲁兰多糖的产量。首先采用单因素试验筛选出有显著效应的3个因素，再利用响应面Box-Behnken设计优化显著因素的水平。结果表明：碳源(蔗糖)添加量、氮源(酵母浸膏)添加量和金属离子对粗普鲁兰多糖的产量都有显著影响(P＜0.05)，蔗糖添加量和酵母浸膏添加量的交互作用相对明显，蔗糖添加量和金属离子以及酵母浸膏添加量和金属离子的交互作用不显著。优化的培养基组成为：蔗糖添加量56.63g/L、酵母浸膏添加量3.74g/L、金属离子选择Mg2+，此条件下粗普鲁兰多糖产量为60.358g/L。

Optimization of Fermentation Medium for Pullulan Production by Response Surface Methodology

The aim of this study is to optimize the fermentation medium for enhancing pullulan production by Aureobasidium pullulans. Important medium components affecting pullulan production were identified by one-factor-at-a-time analysis and further optimized by response surface analysis based on a Box-Behnken design. Sucrose (carbon source) and yeast extract (nitrogen source) concentration and metal ion type had significant effects on pullulan production (P ＜ 0.05). There was a significant interaction effect between sucrose and yeast extract concentration, but no significant interaction effects were observed between metal ion type and sucrose or yeast extract concentration. The optimized fermentation medium was composed of 56.63 g/L sucrose, 3.74 g/L yeast extract and Mg2+, resulting in a pullulan yield of 60.358 g/L.