玉米秸秆生物法制取酒精的中间试验

建立了玉米秸秆采用蒸汽爆破预处理、纤维素酶水解和戊糖己糖同步发酵技术制取酒精的中间试验装置。玉米秸秆在1.6～2.0 MPa条件下蒸汽爆破预处理,在提高玉米秸秆对纤维素酶可及度的同时,玉米秸秆中纤维素、木聚糖和木质素损失分别为4.08%、40.02%和9.91%。里氏木霉以10%的原料制备纤维素酶,并用于降解剩余的90%的原料,滤纸酶活力和纤维素酶水解得率分别为2.27 FPIU/mL和71.3%。初始还原物浓度为43.65 g/L的水解糖液经树干毕赤酵母发酵16 h,还原物利用率和酒精得率分别为87.17%和0.43 g/g(酒精/消耗的糖)。

The Pilot System of Bioethanol Production from Corn Stalks

A pilot system including steam explosion pretreatment,cellulose enzymatic hydrolysis and pentose-hexose simultaneously fermentation was integrated.The cellulose accessibility to cellulase was improved when the corn stalk was pretreated under 1.6～2.0 MPa for 10～15 min,meanwhile the loss of cellulose,xylan and lignin were 4.08%,40.02% and 9.91%,respectively.10% of raw materials were used for cellulase preparation by Trichoderma reesei and was used to hydrolyze the other 90% raw materials in pilot system,the filter paper activity and cellulose hydrolysis yield were 2.27 FPIU/mL and 71.3%,respectively.The hydrolysate containing 43.65 g/L reducing sugars was fermented by Pichia stipitis for 16 h,the consumption of reducing substances and ethanol yield were 87.17% and 0.43 g/g(ethanol/sugar consumption).