| 固定化银杏克隆细胞悬浮培养产生黄酮的研究 |
| |
| 引用本文： | 秦卫东,高明侠,苗敬芝,吕兆启.固定化银杏克隆细胞悬浮培养产生黄酮的研究[J].食品与发酵工业,2005,31(7):45-48. |
| |
| 作者姓名： | 秦卫东 高明侠 苗敬芝 吕兆启 |
| |
| 作者单位： | 徐州工程学院食品工程系,徐州221008 |
| |
| 摘 要： | 采用银杏果实为原料诱导得到颗粒状愈伤组织,在MS培养液中筛选克隆细胞,单细胞在MS+3·0mg/LNAA+1·0mg/LKT中培养。细胞悬浮液与3%的海藻酸钠混合后滴入2%CaCl2MS,制成小球凝胶。固定化细胞在MS+3·0mg/LNAA+1·0mg/LKT+10mg/L肌醇的培养基中悬浮培养25d,黄酮生物合成量为85·63mg/L,克隆细胞增长速度提高24·45倍。
|
| 关 键 词： | 银杏 克隆细胞 固定化 悬浮培养 黄酮 |
| 收稿时间： | 01 5 2005 12:00AM |
| 修稿时间： | 05 23 2005 12:00AM |
Studies on Flavonois Production by Suspended Cultures of Immobilized Cloning Cells of Ginkgo |
| |
| Qin Weidong Gao Mingxia Miao Jingzhi Lv Zhaoqi.Studies on Flavonois Production by Suspended Cultures of Immobilized Cloning Cells of Ginkgo[J].Food and Fermentation Industries,2005,31(7):45-48. |
| |
| Authors: | Qin Weidong Gao Mingxia Miao Jingzhi Lv Zhaoqi |
| |
| Abstract: | In this paper, ginkgo granular callus was obtained and the cloning cells were cloned in the MS medium. 3.0 mg/L NAA and 1.0 mg/L KT were added in MS medium added for the single-cells culture. The suspended solution of cells was mixed with 3% sodium alginate solution, and dripped into MS containing 2% CaCl_2 to form small gelling balls. The immobilized cells were suspended and cultured for 25 days on the medium of MS+3.0mgL NAA+1.0mg/L KT+10mg/L inositol. The amount of biosynthetic flavonoids was 85.63% mg/L and the growth rates of the cloning-cells reached 24.45 times. |
| |
| Keywords: | ginkgo cloning cells immobilization suspended culture flavonoids |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
