马铃薯及其制品中转基因成分的多重PCR检测

采用CTAB法提取15个马铃薯及其制品样品中的总DNA,内源PATA基因的PCR扩增结果均为阳性,表明已提取到DNA。应用根癌农杆菌胭脂碱合成酶基因(nos)终止子和大肠杆菌K12菌株新霉素磷酸转移酶Ⅱ(nptⅡ)基因的二重PCR对样品进行转基因成分检测,结果均为阴性。将马铃薯DNA和阳性质粒pBI121混合作为PCR的反应模板,建立了内源PATA基因、花椰菜花叶病毒(Cauliflowermosaicvirus,CaMV)35S启动子、nos终止子和nptⅡ基因之间的多重PCR检测方法。多重PCR方法具有节约试剂、节省时间等特点,在转基因产品检测上的应用值得推广。

Multiplex PCR Detection of Genetically Modified Components in Potato and Its Products

The total DNAs of fifteen potatoes and their product samples were extracted by CTAB method. The PCR detection results of endogenous PATA gene were all positive, which showed that the DNAs were extracted successfully. The duplex PCR detection results of Agrobacterium tumefaciens nopaline synthase (nos) terminator and Escherichia coli strain K12 neomycin phosphotransferase II (nptⅡ) gene in these samples were all negative. The multiplex PCR detection methods of endogenous PAPA gene, Cauliflower mosaic virus (CaMV) 35S promoter, nos terminator and nptⅡ gene were developed, using the mixture of potato DNA and positive plamid pBI121 as PCR template. The multiplex PCR has some traits such as saving reagent and time, so it may be popularized to detect genetically modified products.