The effect of heat pretreatment temperature on fermentative hydrogen production using mixed cultures |
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| Authors: | Bita Baghchehsaraee George Nakhla Dimitre Karamanev Argyrios Margaritis Gregor Reid |
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| Affiliation: | 1. Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, Ontario N6A 5B9, Canada;2. Department of Microbiology and Immunology, The University of Western Ontario, London, Ontario, Canada;3. Canadian Research and Development Center for Probiotics, Lawson Health Research Institute, 268 Grosvenor Street, London, Ontario N6A 4V2, Canada |
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| Abstract: | The effect of heat treatment at different temperatures on two types of inocula, activated sludge and anaerobically digested sludge, was investigated in batch cultures. Heat treatments were conducted at 65, 80 and 95 °C for 30 min. The untreated inocula produced less amount of hydrogen than the pretreated inocula, with lactic acid as the main metabolite. The maximum yields of 2.3 and 1.6 mol H2/mol glucose were achieved for the 65 °C pretreated anaerobically digested and activated sludges, respectively. Approximately a 15% decrease in yield was observed with increasing pretreatment temperature from 65 to 95 °C concomitant with an increase in butyrate/acetate ratio from 1.5 to 2.4 for anaerobically digested sludge. The increase of pretreatment temperature of activated sludge to 95 °C suppressed the hydrogen production by lactic acid fermentation. DNA analysis of the microbial community showed that the elevated pretreatment temperatures reduced the species diversity. |
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| Keywords: | Biological hydrogen production Heat treatment Inoculum enrichment PCR-DGGE Butyrate/acetate ratio Clostridium |
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