银屑病ILLLI治疗皮肤细胞周期变化的研究

目的:探索银屑病患者ILLLI治疗前后表皮细胞DNA含量的差异。方法:每次ILLLI照射时间1h,每天1次,10次为一疗程。以流式细胞仪检测15例银屑病患者治疗前后及11例正常人表皮细胞的DNA含量,计算细胞周期中各期占整个周期的百分比作对比并作统计学分析。结果:治疗后G1、S期下降,S期接近正常人皮肤值;G2+M 期上升,T检验,P值<0.05,治疗前后有显著差异。S期治疗前与正常皮肤比,P值<0.01,有非常显著差别,治疗后与正常皮肤比,P值>0.05,无差别。结论:银屑病患者ILLLI治疗前后作损害及正常皮肤处表皮细胞周期的分析,证实该疗法能使表皮细胞周期恢复正常,有效治疗银屑病。

Research of Epidermal Cellular Vegetal Cycle of Intravascular Low Level Laser Irradiation in Treatment of Psoriasis

ve: To research epidermal cellular vegetal cycle and the difference of DNA content pre and post intravascular low level laser irradiation (ILLLI) in treatment of psoriasis . Methods : 15 patients suffered from psoriasis were treated by intravascular low level laser irradiation (power:4-5mW,1h per day, a course of treatment 10 days. ) We checked the different DNA content of epidermal cell pre and post treatment as well as 8 natural human. We also calculated the percentage of each phase among the whole cellular cycle and make statistical analysis. Results: The mean value of G1 and S phase decreased obviously while G2+ M phase increased obviously. T test P<0. 05. The related statistical analysis showed significant difference between pre and post treatments. Conclusion: The ILLLI in treatment of psoriasis is effective according to the research of epidermal cellular vegetal cycle and the difference DNA content between pre and post treatment.