Reaction engineering for consecutive enzymatic reactions in peptide synthesis: application to the synthesis of a pentapeptide |
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Authors: | S Ruiz JA Feliu G Caminal G Alvaro J López-Santín |
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Affiliation: | Departament of d'Enginyeria Química, Universitat Autònoma de Barcelona, Bellaterra, Spain. |
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Abstract: | A single-pot enzymatic synthesis of Z-CCK5 (4-8) is presented in this work, employing Z-Gly-Trp-OBzl as acyl donor, under kinetic control. The first goal of the work is the development of a synthetic strategy allowing the use of the same medium for two reactions catalyzed by immobilized alpha-chymotrypsin, discriminating between simultaneous and consecutive addition systems. The second goal is the maximization of the pentapeptide yield as a function of the molar excess of both nucleophiles employed. A maximum yield of 36% was obtained, and the addition strategy as well as the optimal initial concentrations of substrates have been determined. |
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