基于Isobologram方法探究五倍子单宁与Vc、SOD的协同抗氧化作用

以五倍子单宁为主要原料,维生素C(Vc)、超氧化物歧化酶(SOD)为辅料,以1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除、2,2'-联氮-二(3-乙基苯并噻唑啉-6-磺酸)二铵盐(2,2'-azinobis(3-ethylbenzothiazoline...

Isobologram Analysis of Synergistic Antioxidation of Chinese Gallotannins with Vc and SOD

In order to probe the synergistic antioxidant effects of Chinese Gallotannins with Vitamin C(Vc)or Superoxide Dismutase(SOD),Chinese Gallotannins was selected as raw material,Vc and SOD as auxiliary materials,and the antioxidant capacity of Chinese Gallotannins in the presence of Vc or SOD was evaluated by DPPH radical scavenging assay,ABTS radical scavenging assay,Ferricion Reducing Antioxidant Power,Ferric Thocyanant(FTC)method coupled with Isobologram analysis.The results showed that,in the non-oil system,the effect points of the combination of Chinese Gallotannins and Vc,and Chinese Gallotannins with SOD were all distributed below the additive line and 95% confidence limit in the Isobologram analysis diagram,the interaction index(γ)of these compound groups were less-than 1,and the actual IC_(50 mix) value was significantly less than the theoretical IC_(50 add) value.There was a synergistic effect between Chinese Gallotannins with Vc or SOD,The optimal combination proportion of synergetic effect were Chinese Gallotannins:Vc=1∶3 and Chinese Gallotannins:SOD=1∶12 respectively.In the oil system,the oxidation inhibition rates of Chinese Gallotannins,Vc and SOD were 90.19±0.84%,11.84±1.15% and 3.17±0.18% respectively.There was no synergistic effect between Chinese Gallotannins and Vc,and the oxidation inhibition rate was 60.23±1.55%.The combination of Chinese Gallotannins and SOD had synergistic anti-lipid oxidation effect,and the oxidation inhibition rate was 94.58±2.21%,which was 4.39% and 91.41% higher than that of Chinese Gallotannins and SOD,respectively.The results of this work provide fundamental experimental data reference to the development of synergistic antioxidant activities of Chinese Gallotannins.