丝蛋白多肽原位制备金纳米粒子的研究

用来自丝素蛋白的GAGAGAGY序列多肽原位还原氯金酸制备了粒径小于12 nm的球形金纳米粒子,用紫外-可见吸收光谱和透射电子显微镜对反应过程和产物进行了表征分析,探索制备条件对产物的影响。结果表明,多肽可发挥稳定剂的作用,使合成的金纳米溶液具有较好的稳定性;多肽和氯金酸的摩尔比为10:4时合成的粒子粒径大小合适且分布均匀;溶液碱性越强,还原反应速度越慢但金纳米粒径不受影响;反应温度升高,反应速率变快,紫外吸收峰蓝移。

Preparation of Gold Nanoparticles by Using Peptide from Silk Fibroin in situ Reduction

Spherical gold nanoparticles with a particle size of less than 12 nm were prepared by in situ reduction of chloroauric acid with GAGAGAGY, a peptide derived from silk fibroin. The reaction process and product were characterized by UV-Vis absorption spectroscopy and transmission electron microscopy, and the influence of preparative conditions on the product was explored. The results showed that the peptide could act as a stabilizer, endowing the synthesized gold nanoparticles with good stability. When the molar ratio of the polypeptide to chloramic acid is 10 to 4, the size of particles obtained will be uniform. With the increment of alkaline of the solution, the reaction slowed down, but the particle size of the gold nanoparticles was not affected. The rising of temperature could accelerate the reaction, as evidenced by a blue shift of the absorption peak of the reaction solution.