| 产淀粉酶非酿酒酵母菌的筛选及产酶特性研究 |
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| 引用本文： | 徐亚男,刘秋萍,王月,赵九洲,肖婧,史学伟.产淀粉酶非酿酒酵母菌的筛选及产酶特性研究[J].中国酿造,2014(8):90-94. |
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| 作者姓名： | 徐亚男 刘秋萍 王月 赵九洲 肖婧 史学伟 |
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| 作者单位： | 石河子大学食品学院,新疆石河子832000 |
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| 基金项目： | 兵团工业科技攻关项目（2014BA024） |
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| 摘 要： | 通过对非酿酒酵母菌的筛选鉴定,共得到11株产淀粉酶菌株,酶学性质研究表明,该11株酵母菌在pH值为7~9时表现了较高的酶活力,在pH值为5~6时保持稳定;最适反应温度为40℃,在30~50℃时保持较高酶活力;金属离子依赖性发现,Fe2+、Mg2+对酶有不同程度的激活作用,Ca2+、Ba2+、Cu2+对酶有抑制作用;十二烷基硫酸钠(SDS)、乙二胺四乙酸(EDTA)对酶均有抑制作用,EDTA抑制酶活约为50%,淀粉酶对二价金属离子具有一定的依赖作用。
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| 关 键 词： | 非酿酒酵母菌 淀粉酶 酶学性质 |
Screening of amylase-producing non-Saccharomyces yeasts and their characterization |
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| XU Ya＇ nan,LIU Qiuping,WANG Yue,ZHAO Jiuzhou,XIAO Jing,SHI Xuewei.Screening of amylase-producing non-Saccharomyces yeasts and their characterization[J].China Brewing,2014(8):90-94. |
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| Authors: | XU Ya＇ nan LIU Qiuping WANG Yue ZHAO Jiuzhou XIAO Jing SHI Xuewei |
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| Affiliation: | (College of Food Science, Shihezi University, Shihezi 832000, China) |
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| Abstract: | Through the screening of non-Saccharomyces yeasts, 11 amylase-producing strains were screened. Result showed that the yeast showed high enzyme activity at the pH 7-9, stable at pH 5-6. The optimum reaction temperature was 40 ℃, and the yeast was stable at 30-50 ℃. Metal ions dependency experiment indicated that Fe^2＋, Mg^2＋ showed different degree of activation on enzyme. Ca^2＋, Ba^2＋, Cu^2＋, sodium dodecyl sulfate（SDS）, ethylene diamine tetraacetic acid （EDTA） all showed inhibiting effect on enzyme. The inhibition of EDTA was 50% and amylase showed certain dependent on divalent metal ion. |
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| Keywords: | non-Saccharomyces yeasts amylase enzymatic characterization |
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