| 实验用金黄色葡萄球菌生物被膜体外模型的建立 |
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| 引用本文： | 张全凯,;杨公明,;余铭,;杜冰,;吕小丽. 实验用金黄色葡萄球菌生物被膜体外模型的建立[J]. 中国酿造, 2014, 0(5): 53-57 |
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| 作者姓名： | 张全凯, 杨公明, 余铭, 杜冰, 吕小丽 |
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| 作者单位： | [1]华南农业大学食品学院,广东广州510642; [2]阳江职业技术学院生命科学与技术系,广东阳江529500 |
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| 基金项目： | 国家自然科学基金资助项目（31071563） |
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| 摘 要： | 以96孔板为载体,通过改良微孔板法研究细菌接种浓度、培养基pH、培养基浓度、培养温度、培养时间5个单因素对金黄色葡萄球菌生物被膜生长量的影响。在单因素试验基础上,选择培养基pH、培养温度、培养时间3个因素,设计二次回归正交旋转组合试验,建立金黄色葡萄球菌生物被膜实验模型。结果表明,生物被膜成膜最佳条件为细菌接种浓度109CFU/mL、培养基pH7.3、培养基质量分数6%、培养温度37.5℃、培养时间为64.9h,此时96孔板所测光密度理论优化值为3.113 8。
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| 关 键 词： | 金黄色葡萄球菌 生物被膜 体外模型 |
Establishment of experimental in vitro biofilm model of Staphylococcus aureus |
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| Affiliation: | ZHANG Quankai, YANG Gongming, YU Ming, DU Bing, LV Xiaoli (1.College of Food Science, South China Agricultural University, Guangzhou 510642, China; 2.Department of Life Science and Technology, Yangjiang Vocational and Technical College, Yangiiang 529500, China) |
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| Abstract: | The effect of suspension concentration, culture medium pH, medium concentration, culture temperature, culture time on the formation of Staphylococcus aureus biofilms were studied by modified microtiter-plate method using 96 microtitor-plate as the carrier. Based on single factor experiment, the medium pH, culture temperature, and culture time were optimized by quadric regression orthogonal rotational experiment, and the S. aureus biofilm mode was established. The results indicated that the optimal technology was determined as follows： suspension concentration 109 CFU/ml, culture medium pH 7.3, medium concentration 6%, culture temperature 37.5℃, and culture time 64.9 h. Under this optimal condition, the theoretical absorbance of 96 microtitor-plate was 3.1138. |
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| Keywords: | Staphylococcus aureus biofilm in vitro model |
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