反相高效液相色谱法测定刺梨中芦丁和槲皮素的含量

建立反相高效液相色谱法测定刺梨中芦丁和槲皮素的含量。采用WatersSunfire ODS C18色谱柱(4.6 mm×150 mm,5μm),流动相为甲醇-0.2%磷酸缓冲溶液(60:40),流速1.0 mL/min,检测波长360nm,柱温30℃,进样量10μL。结果表明,在上述色谱条件下,芦丁和槲皮素良好分离,质量浓度分别在2~100μg/mL(R2=0.9991)和1~100μg/mL(R2=0.9992)之间线性关系良好。平均加标回收率分别为98.27%和99.15%,相对标准偏差(RSD)分别为2.98%和3.76%,重现性实验RSD分别为芦丁2.18%(n=5),槲皮素1.76%(n=5)。测得刺梨中芦丁和槲皮素的平均含量分别为7.070mg/g和1.352mg/g。RP-HPLC法简便快捷、灵敏,结果准确,可用于刺梨中黄酮成分含量的检测。

Determination of rutin and quercetin in Rosa roxburghii by RP-HPLC

RP-HPLC method for quantitative determination of rutin and quercetin in Rosa roxburghii was established.Waters sunfire ODS C18 column （4.6 mm×150 mm,5μm） was used as fixed phase,and methanol-0.2％ phosphate buffer solution （60：40） was used as mobile phase running with a flow rate of 1.0 ml/min.The detection wavelength was 360 nm and the column temperature was 30 ℃.The results showed that rutin and quercetin were well separated by this method.The rutin and quercetin were in good linear relationship in concentration range of 2-100 μg/ml （R2 =0.999 1） and 1-100 μg/ml （R2 =0.999 2）,respectively.The standard addition recovery was 98.27％ and 99.15％,relative standard deviation was 2.98％ and 3.76％,and the relative standard deviation of reproducibility experiment was 2.18％ （n=5） and 1.76％ （n=5）,respectively.The average content of rutin and quercetin in Rosa roxburghii were 7.07 mg/g and 1.352 mg/g.The RP-HPLC method was simple,quick,accurate and sensitive,which can be used in rutin and quercetin content determination.