| 米曲霉不同培养方式的发酵探究 |
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| 引用本文： | 肖君,;甄玉国,;王晓磊.米曲霉不同培养方式的发酵探究[J].中国酿造,2014(7):48-50. |
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| 作者姓名： | 肖君 ;甄玉国 ;王晓磊 |
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| 作者单位： | [1]吉林农业大学动物科学技术学院吉农博瑞奶牛研发中心,吉林长春130118; [2]长春博瑞饲料集团有限公司技术中心,吉林长春130114 |
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| 基金项目： | 吉林省重大科技攻关项目资助（2012ZDGG006） |
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| 摘 要： | 利用10L发酵罐在摇瓶实验基础上进行了米曲霉(Aspergillusoryzae)放大实验。根据不同菌体形态对代谢产物的影响,在米曲霉菌丝生长到絮状前,终止发酵。指数补料实验结果表明,分批发酵在第14小时菌体干质量达到最大值15.06g/L,比摇瓶发酵提高了33%;指数补料在第16小时菌体干质量达到20.43g/L,比分批发酵提高26%。
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| 关 键 词： | 米曲霉 发酵罐 指数补料 |
Exploration of Aspergillus oryzae fermentation with different cultivate modes |
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| Affiliation: | XIAO Jun,ZHEN Yuguo,WANG Xiaolei(JAU-Borui Dairy Science and Technolgy R ＆ D Center, College Animal Science ＆ Technology, Jilin Agricultural University,Changchun 130118,China;JAU-Borui Dairy Science and Technolgy R ＆ D Center,College Animal Science ＆ Technology, Jilin Agricultural University,Changchun 130118,China;Technolgy Center of Changchun Borui Feed Co., Ltd., Changchun 130114, China) |
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| Abstract: | Based on the shake flasks results scaling up experiment of Aspergillus oryzae in 10 L fermentor was conducted.Accroding to the effect of different m ycelialm orphology on them etabolite,ferm enting should be stopped before A.oryzae hypha growing into flocculent The exponential feeding results show ed thathe celldry w eightcould reach 15.06 g/L when cultivated by batch fermentation for 14 h,which w as33％ higher than shake flask culture.A the 16 h of fermentation,the celldry weight was 20.43 g/L by exponential feeding,which w as26％ higher than batch ferm entation. |
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| Keywords: | Aspergillus oryzae fermentor exponential feeding |
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