Effects of sampling site on passage rate estimates in heifers fed alfalfa hay or a high concentrate diet

Rate of passage from the rumen was estimated from samples from the rumen, duodenum, ileum, and rectum after four crossbred heifers were dosed with cobalt ethylenediaminetetraacetic acid and ytterbium-labeled alfalfa or corn grain as digesta markers. Diets consisted of chopped alfalfa hay or an 80% concentrate mixture fed twice daily at 2.0% of body weight (dry matter per day) in two trials. Rates of particulate passage were calculated by two mathematical models, and influence of site of sampling on rate of ruminal passage was appraised. Rate of fluid passage calculated from ruminal samples was more rapid than rates from samples at the duodenum, ileum, and rectum for both the alfalfa diet (10.6 versus 6.3, 5.6, and 5.6% X h-1) and the 80% concentrate diet (10.3 versus 6.8, 7.2, and 6.1% X h-1). With the alfalfa diet, rates of fluid passage estimated from ruminal and rectal samples were correlated .98 despite the marked disparity of means. Rates of passage of particulates estimated from samples from the duodenum, ileum, and rectum were not correlated. Estimates of rate of digesta passage from the rumen differed with postruminal site of sampling.