| 麻竹叶与陈皮中总黄酮分离及含量测定 |
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| 引用本文： | 李志海,王远强,刘鉴,林治华. 麻竹叶与陈皮中总黄酮分离及含量测定[J]. 重庆理工大学学报(自然科学版), 2008, 22(7) |
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| 作者姓名： | 李志海 王远强 刘鉴 林治华 |
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| 作者单位： | 重庆工学院化学与生物工程学院 重庆400050 |
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| 摘 要： | 用超临界CO2萃取方法提取麻竹叶和陈皮中的黄酮化合物,用乙醇萃取得萃取物.利用紫外分光光度法,以芦丁为标准品,在紫外最大吸收峰510 nm处测定乙醇萃取物中总黄酮的含量.麻竹叶、陈皮中的总黄酮的含量分别为1.36%、2.48%.试验表明,该方法简便、快速、重现性好,可作为检测麻竹叶和陈皮中总黄酮含量的一种手段.
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| 关 键 词： | 黄酮 超临界CO2萃取 芦丁 紫外分光光度法 |
Extracting and Assaying the Total Flavonoid from Numb Bamboo and Dried Tangerine Peel |
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| LI Zhi-hai,WANG Yuan-qiang,LIU Jian,LIN Zhi-hua. Extracting and Assaying the Total Flavonoid from Numb Bamboo and Dried Tangerine Peel[J]. Journal of Chongqing University of Technology(Natural Science), 2008, 22(7) |
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| Authors: | LI Zhi-hai WANG Yuan-qiang LIU Jian LIN Zhi-hua |
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| Abstract: | Supercritical CO2 fluid extraction(SFE-CO2) is used as a method to extract flavonoid from numb bamboo and dried tangerine peel with ether.Making use of ultraviolet spectrophotometry and taking Rutin as a standard article,the total flavonoid in the ether at the 510 nm where is the biggest absorption is measured.The total flavonoid from numb bamboo and dried tangerine peel are 1.36%and 2.48% respectively.Experiments show that the method is simple,fast,repeatable,being a means to measure the total flavonoid from the nump bamboo and dried tangerine peel. |
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| Keywords: | flavonoid Supercritical CO2 fluid extraction(SFE-CO2) Rutin ultraviolet spectrophotometry |
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