Uncharacterized Escherichia coli proteins YdjA and YhjY are related to biohydrogen production |
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| Authors: | Mohd Zulkhairi Mohd Yusoff Toshinari Maeda Viviana Sanchez-Torres Hiroaki I. Ogawa Yoshihito Shirai Mohd Ali Hassan Thomas K. Wood |
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| Affiliation: | 1. Department of Biological Functions and Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0196, Japan;2. Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia;3. Department of Chemical Engineering & Biochemistry and Molecular Biology, Pennsylvania State University, 161 Fenske Laboratory, University Park, PA 16802, USA |
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| Abstract: | Biohydrogen has gained importance as an alternative energy source, and advances in molecular biology and biotechnology have raised the quality and efficiency of biohydrogen production from various microorganisms and substrates. Here, Escherichia coli proteins YdjA and YhjY have been identified as essential in biohydrogen production from glucose. The mutations ydjA and yhjY reduced biohydrogen productivity compared to the parent strain from 40 to 4 and 29 μmol/mg protein, respectively. Through transcription analysis, it was determined that YdjA and YhjY are positive effectors of the FHL complex since their inactivation repressed fhlA. In addition, the FHL expression of the repressor gene, hycA, increased for the ydjA mutant, so YdjA reduces transcription of the HycA repressor. Hence, two new proteins have been identified that are important for biohydrogen production. |
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| Keywords: | Biohydrogen Escherichia coli ydjA yhjY FHL inactivation |
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