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Effects of linoleic acid and its degradation by-products on mesophilic hydrogen production using flocculated and granular mixed anaerobic cultures |
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| Authors: | Noori M. Cata Saady Subba Rao Chaganti Jerald A. Lalman Sathyanarayanan S. Veeravalli Saravanan R. Shanmugam Daniel D. Heath |
| Affiliation: | 1. Department of Civil and Environmental Engineering, University of Windsor, 205 Essex Hall, 401 Sunset Avenue, Windsor, ON, Canada N9B 3P4;2. Great Lakes Institute for Environmental Research, University of Windsor, 401 Sunset Avenue, Windsor, ON, Canada N9B 3P4;3. Department of Biological Sciences, Room 209, Great Lakes Institute for Environmental Research, University of Windsor, 401 Sunset Avenue, Windsor, ON, Canada N9B 3P4 |
| Abstract: | The effects of linoleic acid (LA (C18:2)) and its degradation by-products on hydrogen (H2) production were examined at 37 °C and an initial pH value of 5.0 using granular and flocculated mixed anaerobic cultures from the same source. In the flocculated cultures, the H2 consumers were inhibited to a greater extent when compared to the granular cultures. The maximum H2 yields were 2.52 ± 0.2 and 1.9 ± 0.2 mol mol−1 glucose in the flocculated and granular cultures, respectively. The major long chain fatty acids (LCFAs) detected at which H2 attained a maximum value were LA (750 mg L−1) and myristic acid (MA) (500 mg L−1). |
| Keywords: | Biohydrogen Linoleic acid (LA (C18:2)) Anaerobic mixed culture Principal component analysis (PCA) Flux balance analysis T-RFLP |
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