Enhancement of hydrogen production by the filamentous non-heterocystous cyanobacterium Arthrospira sp. PCC 8005 |
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| Authors: | Wuttinun Raksajit Khomson Satchasataporn Kirsi Lehto Pirkko Mäenpää Aran Incharoensakdi |
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| Affiliation: | 1. Center of Integrated Bioscience for Animal Health and Alternative Energy, Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand;2. Department of Biochemistry and Food Chemistry, Laboratory of Molecular Plant Biology, FI20014 Turku, Finland;3. Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand |
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| Abstract: | The efficiency of hydrogen production by different cyanobacterial species depends on several external factors. We report here the factors enhancing hydrogen production by filamentous non-heterocystous cyanobacterium Arthrospira sp. PCC 8005. Cells adapted to dark-anaerobic conditions produced hydrogen consistent with increased hydrogenase activity when supplemented with Fe2+. Stimulation of hydrogen production could be achieved by addition of reductants, either dithiothreitol or β-mercaptoethanol with higher production observed with the latter. Additionally, Fe2+ and β-mercaptoethanol added to nitrogen- and sulphur-deprived cells significantly stimulated H2 production with maximal value of 5.91 ± 0.14 μmol H2 mg Chla−1 h−1. Glucose and a small increase of osmolality imposed by either NaCl or sorbitol enhanced hydrogen production. High rates of hydrogen production were obtained in cells adapted in nitrogen-deprived medium with neutral and alkaline external pH, significant decrease of hydrogen production occurred under acidic external pH. |
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| Keywords: | Arthrospira sp PCC 8005 Hydrogen production Hydrogenase Iron supplementation Reductant Nitrogen and sulphur deprivation |
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