普鲁卡因对光动力作用的影响

目的:1)体外观察光敏剂CPD5对人胰癌细胞(Hs766T)的光动力效果;2)观察普鲁卡因对CPD5光动力作用的影响.方法:浓度为1×105/ml的胰癌细胞,加到96孔培养板中,每孔100 μl,然后再加入不同浓度的CPD5,培养24 h弃旧液,更换新鲜培养液.普鲁卡因组加普鲁卡因,CPD5光动力对照组加生理盐水.670 nm半导体激光器,光剂量10 J/cm2,逐孔照射3 min.继续培养24 h后,用细胞排染试验测细胞存活率.结果:1)CPD5浓度为2.5μg/ml时,PDT后胰癌细胞存活率为2.0%;2)加入普鲁卡因(20 μg/ml)后,CPD5(浓度为2.5μg/ml)光动力效果是胰癌细胞存活率97.0%.结论:普鲁卡因可明显地淬灭CPD5光动力作用.

Effect of Procaine on photodynamic therapy

Objective: ① Observe from outside the body the photodynamic therapy of light sensitive agent CPD5 on human pancreas cancer cells(Hs766T);② Observe the light-generated effect of Procaine on CPD5. Method: add pancreas cancer cells with a concentration of 1×105 ml into a culture panel with 96 holes,each hole 100 μl,then add in CPD5 of different concentrations,replace the used cultures with fresh ones 24 hours later. Add Procaine to the Procaine group and physiological saline to the light-generated compare group. Expose each hole to a radiation of 670 nm laser diode with a dose of 10 J/cm2 for 3 minutes. Measure the livability of cells with the dyeing cell experiment after another 24 hours of culture. Result:①The livability of pancreas cancer cells after the PDT is 2.0% when the concentration of CPD5 is at 2.5 μg/ml. ② The light-generated effect of CPD5 with a concentration of 2.5 μg/ml after the addition of Procaine(20 μg/ml)is the livability of pancreas cancer cells is 97.0%.Conclusion: Procaine can greatly decrease the light-generated effect of CPD5.