| 超声波辅助水酶法萃取葫芦籽油的研究 |
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| 引用本文： | 方芳.超声波辅助水酶法萃取葫芦籽油的研究[J].中国粮油学报,2012,27(10):62-66. |
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| 作者姓名： | 方芳 |
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| 作者单位： | 长沙理工大学化学与生物工程学院,长沙,410114 |
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| 基金项目： | 国家自然科学基金(51104024);湖南省自然科学基金(10JJ6019);湖南省教育厅项目(10C0359);湖南省科技厅项目(2009GK3024) |
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| 摘 要： | 以葫芦籽粉为原料,采用水酶法和超声波辅助水酶法萃取葫芦籽油,并对其中的酶解条件和超声波预处理条件进行研究,经单因素试验与正交试验,确定水酶法萃取葫芦籽油的适宜酶解条件为:料液比1∶8,pH9.0,酶解温度55℃,酶解时间4h,酶用量2.5％,在此条件下葫芦籽油萃取率为79.9％.水酶法提油前对葫芦籽粉进行超声波预处理,可有效提高葫芦籽油的萃取率.在超声波温度55℃,超声波功率500W下处理6min可将葫芦籽油萃取率提高至88.5％,比未经超声波预处理的高出8.5％.
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| 关 键 词： | 葫芦籽油 水酶法 超声波 正交试验 |
| 收稿时间： | 2011/12/27 0:00:00 |
| 修稿时间： | 2012/3/30 0:00:00 |
Ultrasound- assisted aqueous enzymatic extraction of Cucurbit Seed Oil |
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| Fang Fang
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Xu Kaiyang
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Zhu Qiang
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Shen Zhicheng.Ultrasound- assisted aqueous enzymatic extraction of Cucurbit Seed Oil[J].Journal of the Chinese Cereals and Oils Association,2012,27(10):62-66. |
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| Authors: | Fang Fang Xu Kaiyang Zhu Qiang Shen Zhicheng |
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| Affiliation: | Fang Fang Xu Kaiyang Zhu Qiang Shen Zhicheng(School of Chemistry and Biological Engineering Changsha University of Science and Technology,Changsha 410114) |
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| Abstract: | Aqueous enzymatic extraction of cucurbit seed oil from cucurbit seed powder assisted by ultrasound was studied,and the enzymatic hydrolysis conditions were optimized emphatically.The hydrolysis conditions were determined as follows:ratio of stuff to water 1∶ 8,pH 9.0,temperature 55 ℃,time 4 h,dosage of alcalase 2.5%.Under the optimal conditions,the oil extraction efficiency reached 79.9%.The ultrasound irradiation at 500W and 55 ℃ for 6 min followed by aqueous enzymatic extraction could increase the oil extraction efficiency to 88.5%,which was 8.5% higher than that of non-irradiation with ultrasound. |
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| Keywords: | cucurbit seed oil aqueous enzymatic extraction ultrasound orthogonall experiment |
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