天然燕麦肽的提取工艺优化及生物活性初探

以山西产裸燕麦为原料,采用单因素和二次回归旋转组合试验研究了不同因素对燕麦肽得率的影响,确定了天然燕麦肽提取的最佳工艺参数,并探讨了燕麦肽的生物活性。试验结果表明,在试验范围内各因素对燕麦肽得率的影响程度由大到小依次为:提取温度、料液比、提取时间。提取燕麦肽的最优工艺参数为液料比16∶1(mL/g),提取时间72 min,提取温度34℃。在此条件下,试验得到的燕麦肽得率为5.54 mg/g。生物活性试验结果表明,天然燕麦肽具有一定的抗氧化和降血压活性。

Primary Study on Extracting Technology Optimization and Biological Activity of Peptides from Natural Oat Groats

Taking the naked oat seeds as raw materials,the effects of different factors on peptide yield and optimum parameters for extraction technology of natural peptide from oat groats were determined by the single-factor experiment and quadratic orthogonal rotation regression combination design and the bioactivity of peptides was investigated.The result showed that the effect sequences of three factors for peptide yield were as follows: extraction temperature,liquid-to-solid ratio,and extraction time.The optimal extraction parameters were as follows: liquid-to-solid ratio of 16∶ 1(mL/g),extraction time of 72 min,and extraction temperature of 34 ℃.Under the said conditions,the yield of peptide from oat groats was 5.54 mg/g.The biological activity test exhibited that the oats had certain antioxidant activity and effect of reducing blood pressure.