| 甜菜碱对溶菌酶去折叠热力学和复性动力学的影响 |
| |
| 引用本文： | 刘建华,董晓燕,武扬,孙彦.甜菜碱对溶菌酶去折叠热力学和复性动力学的影响[J].化工学报,2007,58(2):465-470. |
| |
| 作者姓名： | 刘建华 董晓燕 武扬 孙彦 |
| |
| 作者单位： | 天津大学化工学院生物化工系 天津大学化工学院生物化工系 天津大学化工学院生物化工系 天津大学化工学院生物化工系 |
| |
| 基金项目： | 河北省教育厅科研项目
,
唐山市科学技术研究和发展计划 |
| |
| 摘 要： | 应用荧光分析技术考察了甜菜碱对溶菌酶在盐酸胍溶液中稳定性的影响；并提出将甜菜碱作为添加剂应用于彻底变性还原溶菌酶的复性；同时应用表观竞争反应动力学模型分析了不同浓度甜菜碱对溶菌酶复性动力学的影响特性。结果表明，甜菜碱对溶菌酶具有稳定作用，可以作为添加剂促进变性还原溶菌酶的复性。甜菜碱可以抑制复性过程溶菌酶分子间的聚集，同时提高溶菌酶的复性速率，从而提高溶菌酶的复性收率。
|
| 关 键 词： | 溶菌酶 去折叠 复性 动力学 甜菜碱 |
| 文章编号： | 0438-1157（2007）02-0465-05 |
| 收稿时间： | 2006-3-13 |
| 修稿时间： | 2006-03-132006-08-28 |
Effect of betaine on unfolding thermodynamics and refolding kinetics of lysozyme |
| |
| LIU Jianhua,DONG Xiaoyan,WU Yang,SUN Yan.Effect of betaine on unfolding thermodynamics and refolding kinetics of lysozyme[J].Journal of Chemical Industry and Engineering(China),2007,58(2):465-470. |
| |
| Authors: | LIU Jianhua DONG Xiaoyan WU Yang SUN Yan |
| |
| Affiliation: | Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China |
| |
| Abstract: | Equilibrium denaturation of lysozyme by guanidinium chloride in the presence of betaine was investigated by tryptophan fluorescence. Betaine was used as a folding aid to enhance the renaturation of denatured-reduced lysozyme, the refolding kinetic behavior was studied at a low guanidinium chloride concentration by a competitive model of first-order folding reaction and third-order aggregation. It was found that betaine could shift in the transition midpoint in the guanidinium chloride induced equilibrium unfolding experiments, indicating that betaine could improve the thermodynamic stability of lysozyme. In the presence of betaine, the aggregation rate was decreased and the refolding rate was increased, the refolding yield could be increased. |
| |
| Keywords: | lysozyme unfolding refolding kineties betaine |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《化工学报》浏览原始摘要信息 |
|
点击此处可从《化工学报》下载全文 |
