Mechanisms behind tailing in the pressure inactivation curve of a clinical isolate of Escherichia coli O157:H7 |
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Authors: | Noma Seiji Kajiyama Daiki Igura Noriyuki Shimoda Mitsuya Hayakawa Isao |
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Affiliation: | Kyushu University Venture Business Laboratory, 6-10-1, Hakozaki, Higashi-ku, Fukuoka City, 812-8581, Japan. |
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Abstract: | The tailing in pressure inactivation curve of clinically isolated Escherichia coli O157:H7 was investigated. A typical tailing was observed after the treatment period for 30min when 10(7) CFU/ml of the cell suspension was subjected to pressure treatment at 300MPa and 25 degrees Celsius. There was no effect on the tailing profiles by the addition of pressure-killed cells and released cellular components. When cells survived at a tail portion were re-propagated (tail-culture) and subjected to second pressure treatment, the cells of the tail-culture exhibited eminently higher barotolerance compared to those of the original-culture, suggesting that the presence of genetically pressure-resistant subpopulation was responsible for the tailing. The cytoplasmic membrane of the tail-culture cells had higher stability to a pressure treatment at 100MPa for 10min than that of the original-culture, which was evidenced by lower permeability to ethidium bromide. The addition of non-ionic surfactants including 0.5microl/ml polyoxyethylene p-t-octylphenyl ester (Triton X-100) and 0.53mg/ml lauric sugar ester dramatically reduced the level of tailing and made the inactivation curve linear. |
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