拮抗细菌活性蛋白诱导烟草对CMV的抗性研究

从烟田耕作层土样中分离到对CMV有拮抗作用的细菌菌株B6，通过离子交换层析和凝胶过滤层析初步确定其活性物质是分子质量大小为40．6kD的蛋白质。活性蛋白诱导后，对烟草叶片的病毒含量、过氧化物酶（POD）、苯丙氨酸解氨酶（PAL）和多酚氧化酶（PPO）的活性变化进行研究。结果表明，拮抗细菌活性蛋白诱导后烟草叶片内CMV含量明显降低，随着诱导天数的增加病毒的含量呈由多到少再增多的变化趋势，POD，PAL和PPO等细胞防御酶的活性增强。

Induced Resistance to CMV in Tobacco Treated with Antagonistic Bacteria Active Protein

The virus disease is one of the major diseases on crops.The control effects and security of antiviral agent are not as good as people's demands till now.New antiviral agents are in dire need of researching.In this study,we isolated bacterial strain B6 from healthy and sick tobacco soil.The results presented here demonstrated the active material was protein through using cation exchange chromatography and molecular sieve chromatography.The relative molecular mass of subunit was 40.6 kD.The induced resistance to CMV in tobacco was treated by antiviral protein.,and the CMV contents and the activity of PPO,POD,PAL were studied.The results showed that with the time extending of inducing,the content of CMV increased at beginning,and then decreased,at last increased again.Antiviral protein could enhance the activity of cytophylaxis enzyme such as PPO,POD and PAL.