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Incorporation of [1-14C] acetate into phospholipids by soybean seedlings
Authors:T. Negishi
Affiliation:(1) Department of Agricultural Chemistry, Obihiro Zootechnical University, Obihiro, Hokkaido, Japan
Abstract:Incorporation of [1-14C] acetate into lipids by slices of soybean seedlings was studied. The results were as follows: (a) the greatest amount of radioactivity was detected in the phospholipid fraction prepared from the main axis; (b) in the cotyledons, radioactivity was about the same in pigment, phosphatidylethanolamine, and phosphatidylglycerol; (c) phosphatidylcholine was the main phospholipid labeled in the axis; (d) the distribution of radioactive fatty acids in the axis suggested that this tissue has the capacity for both phospholipid synthesis and fatty acid desaturation.
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