Evaluation of the biocompatibility of a new vascular prosthesis coating by detection of prosthesis-specific antibodies |
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Authors: | Uwe Walschus Helmut Goldmann Torsten Ueberrueck Andreas Hoene Lutz Wilhelm Michael Schlosser |
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Affiliation: | (1) Research Group of Predictive Diagnostics, Department of Medical Biochemistry and Molecular Biology, Ernst Moritz Arndt University Greifswald, Greifswalder Str. 11c, 17495 Karlsburg, Germany;(2) Institute of Pathophysiology, Ernst Moritz Arndt University Greifswald, Karlsburg, Germany;(3) AESCULAP AG & Co. KG, Tuttlingen, Germany;(4) Department of Surgery, University of Jena, Jena, Germany;(5) Department of Surgery, Ernst Moritz Arndt University Greifswald, Greifswald, Germany;(6) Department of Surgery, Hospital Demmin, Demmin, Germany |
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Abstract: | In recent experimental studies, we could demonstrate the occurrence of antibodies against the prosthesis matrix and coating
following implantation of polyester-based vascular grafts. Therefore, this study aimed at evaluating the biocompatibility
of a new absorbable polymer coating by detection of antibodies against the coating and the polyester matrix. Two polyester
vascular prostheses coated either with the polymer (PP-prosthesis) or with gelatine (PG-prosthesis) were functionally implanted
into sheep (n = 22 per group). Blood was drawn on days 1 (pre-OP) and 7, 14, 28, 56, 84, 140 (post-OP). Homogenates from both prostheses
(PP-target or PG-target) or from an uncoated prosthesis (P-target) were used as assay targets in a particle-based immunoassay.
The antibody binding against the P-target was significantly higher in the PP-group than in the PG-group on days 7–56, but
not on days 84 and 140. Within both groups, no significant differences but a significant correlation between the binding against
the P-target and the coated target was found. Therefore, the absorbable polymer did not induce a specific humoral immune response.
In conclusion, the overall immunogenicity of the polymer-coated graft was comparable to the gelatine-coated graft. The detection
of prosthesis-specific antibodies seems to be useful for in vivo biocompatibility testing. |
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