Identification and Field Evaluation of Sex Pheromone Components of the Pear Barkminer Moth, <Emphasis Type="Italic">Spulerina astaurota</Emphasis> |
| |
Authors: | Nguyen Duc Do Kanako Ohbayashi Hideshi Naka Ken Nakada Tetsu Ando |
| |
Affiliation: | (1) Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, Koganei Tokyo, 184-8588, Japan;(2) Laboratory of Applied Entomology, Faculty of Agriculture, Tottori University, 4-101, Koyama-minami, Tottori Tottori, 680-8553, Japan;(3) Horticultural Research Center, Tottori Prefectural Agriculture and Forest Research Institute, 2048 Yura-shuku, Hokuei Tottori, 689-2221, Japan; |
| |
Abstract: | The pear barkminer moth, Spulerina astaurota Meyrick (Gracillariidae: Gracillariinae), is a harmful pest of the Asian-pear tree. Pheromone components of the female were
analyzed by gas chromatography (GC) with an electroantennographic (EAG) detector and GC coupled with mass spectrometry. The
analyses of a crude pheromone extract and those of a fractionated extract on a Florisil column indicated three EAG-active
components, tetradecadien-1-ol, its acetate, and an aldehyde derivative. Characteristic fragment ions in the mass spectra
of the dienyl compounds and derivatives with 4-methyl-1,2,4-triazoline-3,5-dione revealed double bonds at the 9- and 11-positions.
By comparing the chromatographic behaviors to those of four authentic geometrical isomers, which were synthesized by three
different routes starting from 1,8-octanediol or 1,9-nonanediol, the configuration of each natural component was assigned
to be 9Z,11Z; i.e., it was concluded that the S. astaurota females secreted (9Z,11Z)-9,11-tetradecadien-1-ol (Z9,Z11-14:OH) as a main pheromone component, and the acetate and aldehyde derivatives (Z9,Z11-14:OAc
and Z9,Z11-14:Ald) as minor components. This identification was confirmed by a field evaluation of the synthetic pheromone.
While the male moths could be attracted to a lure baited with Z9,Z11-14:OH alone, Z9,Z11-14:OAc showed a strong synergistic
effect on the attraction. Among the lures tested, the mixture of alcohol and acetate in a ratio of 7:3 exhibited the strongest
attraction. Addition of Z9,Z11-14:Ald in the mixture did not significantly increase the number of males attracted. Furthermore,
the field test indicated that some contamination of a geometrical isomer of the alcohol did not impair the activity of the
binary mixture with the 9Z,11Z configuration. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|