Tunable Nanoparticle and Cell Assembly Using Combined Self‐Powered Microfluidics and Microcontact Printing |
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Authors: | Cyrille Hamon Malou Henriksen‐Lacey Andrea La Porta Melania Rosique Judith Langer Leonardo Scarabelli Ana Belén Serrano Montes Guillermo González‐Rubio Marian M de Pancorbo Luis M Liz‐Marzán Lourdes Basabe‐Desmonts |
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Affiliation: | 1. Bionanoplasmonics Laboratory, CIC biomaGUNE, Donostia–San, Sebastian, Spain;2. CIBER de Bioingeniería, Biomateriales y Nanomedicina, CIBER‐BBN, Madrid, Spain;3. BIOMICs Research Group, Lascaray Ikergunea Research Center, University of the Basque Country (UPV/EHU), Vitoria‐Gasteiz, Spain;4. Ikerbasque, Basque Foundation of Science, Bilbao, Spain;5. BIOMICs microfluidics Research Group, Microfluidics Cluster UPV/EHU, Lascaray Ikergunea Research Center, University of the Basque Country (UPV/EHU), Vitoria‐Gasteiz, Spain |
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Abstract: | The combination of cell microenvironment control and real‐time monitoring of cell signaling events can provide key biological information. Through precise multipatterning of gold nanoparticles (GNPs) around cells, sensing and actuating elements can be introduced in the cells' microenviroment, providing a powerful substrate for cell studies. In this work, a combination of techniques are implemented to engineer complex substrates for cell studies. Alternating GNPs and bioactive areas are created with micrometer separation by means of a combination of vacumm soft‐lithography of GNPs and protein microcontract printing. Instead of conventional microfluidics that need syringe pumps to flow liquid in the microchannels, degas driven flow is used to fill dead‐end channels with GNP solutions, rendering the fabrication process straightforward and accessible. This new combined technique is called Printing and Vacuum lithography (PnV lithography). By using different GNPs with various organic coating ligands, different macroscale patterns are obtained, such as wires, supercrystals, and uniformly spread nanoparticle layers that can find different applications depending on the need of the user. The application of the system is tested to pattern a range of mammalian cell lines and obtain readouts on cell viability, cell morphology, and the presence of cell adhesive proteins. |
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Keywords: | cell assemblies degas driven flow gold nanoparticles microcontact‐printing patterning |
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