Mathematical modeling and optimization of plasmid-harboring and chromosome-integrated recombinant yeast culture processes |
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Authors: | Hyung Joon Cha Young Je Yoo |
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Affiliation: | (1) Department of Chemical Engineering and The Institute for Molecular Biology and Genetics, Seoul National University, 151-742 Seoul, Korea |
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Abstract: | For the enhanced secretion of foreign proteins, the mathematical modeling for overall recombinant yeast culture considering
protein secretion dynamics is important because we can understand and predict the behavior of biosynthesis and secretion of
foreign protein and can develop control strategies for feeding with these model equations. In this research, the mathematical
modeling and simulation considering protein secretion dynamics for recombinant yeast cultures that contain multicopy plasmids
or chromosomally integrated genes were performed for optimization of foreign glucoamylase production. The optimal feeding
policy for maximizing glucoamylase production was suggested in fed-batch culture. By introducing this optimal feeding policy,
final glucoamylase activity and productivity of fedbatch culture were significantly increased compared with those of batch
culture in both recombinant yeasts. |
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Keywords: | Plasmid-Harboring Recombinant Yeast Chromosome-Integrated Recombinant Yeast Glucoamylase Mathematical Modeling Optimization |
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