| 啤酒废酵母核糖核酸提取过程中去除蛋白质的研究 |
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| 引用本文： | 王晓丽,郑飞云,李崎,顾国贤. 啤酒废酵母核糖核酸提取过程中去除蛋白质的研究[J]. 中国酿造, 2006, 0(3): 27-30 |
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| 作者姓名： | 王晓丽 郑飞云 李崎 顾国贤 |
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| 作者单位： | 江南大学工业生物技术教育部重点实验室,江苏,无锡,214036 |
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| 摘 要： | 讨论了3种去除核糖核酸（RNA）中蛋白质的方法，通过对蛋白质去除效果及影响RNA损失情况的分析比较，发现添加40U／g绝干酵母中性蚩白酶降解蛋白质的方法为最好，这种方法制得RNA粗品，得率达到5．75％，蛋白质去除了96．6％。
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| 关 键 词： | 啤酒废酵母 蛋白质 提取 |
| 文章编号： | 0254-5071(2006)03-0027-04 |
| 修稿时间： | 2005-09-28 |
Removal of protein in RNA extraction of waste brewing yeast |
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| WANG Xiao-li,ZHENG Fei-yun,LI Qi,GU Guo-xian. Removal of protein in RNA extraction of waste brewing yeast[J]. China Brewing, 2006, 0(3): 27-30 |
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| Authors: | WANG Xiao-li ZHENG Fei-yun LI Qi GU Guo-xian |
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| Abstract: | Three methods of removing proteins in RNA were discussed in this paper.The best way was to add 40 U neutral protease per gram dry yeast,and to compare the effects of protein removal to the loss of RNA.The extraction yield of this crude RNA product was 5.75% while the removal ratio of protein was 96.6%. |
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| Keywords: | RNA |
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