刺葡萄皮色素动态吸附解吸纯化工艺研究

对HP-20大孔树脂纯化刺葡萄皮色素的特性进行了研究,得到刺葡萄皮色素动态吸附及解吸适宜的工艺参数。结果表明,上样液pH3,流速4.5BV/h,色素液浓度1.5891～3.1377mg/ml可获得较好的吸附效果;80%乙醇(含0.05%盐酸)是刺葡萄皮色素适宜的洗脱剂,洗脱速度控制为3BV/h,洗脱剂用量8BV可获得最佳解吸效果。在以上优化动态吸附解吸工艺条件下,HP-20树脂对刺葡萄皮色素动态饱和吸附量达到39.0mg/ml湿树脂,解吸率达到92%以上,色素液经纯化后,其色价为原来的5.93倍,取得理想的纯化效果。

Study on Purification Technique of Vitis davidii Forx.Skin Pigment under Dynamic Adsorption and Desorption Conditions

The purification characteristics of macroporous resin HP-20 to Vitis davidii Foex. skin pigment(VSP) were determined in this study,and the technical parameters of the dynamic adsorption and desorption were optimized. The results showed that the optimal dynamic adsorption conditions are as follows:pH of crude VSP sample 3,its flow rate 4.5 BV/h,and its concentration 1.5891 to 3.1377 mg/ml. 80% Ethanol(containing 0.05% HCl) is the optimum eluent for desorpting VSP,and its optimal folw rate and amount for elution are 3 BV/h and 8 BV,respectively. Under the optimized technical conditions of dynamic adsorption and desorption mentioned above,the resin HP-20 has the saturation adsorption amount of 39.06 mg/ml(wet resin) ,and its desorption rate is more than 92%. The colour value of purified VSP slution is 5.93 times as much as that of unpurified one.