星点设计-效应面法优化白芷超临界CO2萃取工艺

采用星点设计-效应面法优化白芷超临界CO2萃取工艺。以萃取温度、夹带剂浓度和萃取时间为自变量,以总香豆素得率为因变量对自变量各水平进行多元线性回归和二项式拟合,用效应面法选取最佳工艺条件,并进行预测分析。得到的最佳萃取条件为:萃取温度54℃、夹带剂浓度43%、萃取时间97 min,在此条件下总香豆素得率可达0.212%,与预测值的偏差为-2.42%。利用星点设计-效应面法优化白芷萃取工艺,方法简便,预测性良好。

Optimization of Supercritical CO2 Extraction Process of Angelica Dahurica by Central Composite Design/Response Surface Methodology

The supercritical CO2 extraction process of Angelica dahurica was optimized by central composite design/response surface methodology.Linear or no-linear mathematic models were performed with extraction temperature,modifier concentration and extraction time as independent variables and extraction rate of total coumarins as dependent variable.Response surface methodology was used to optimize the extraction process and to predict the values.The optimal extraction conditions were as follows： extraction temperature 54℃,modifier concentration 43% and extraction time 97 min.Under these conditions,extraction rate of total coumarins could reach 0.212%.Bias between observed and predicted values was-2.42%.Central composite design/response surface methodology is convenient and highly predictive for optimizing the extraction process of Angelica dahurica.