DNA recognition by a {beta}-sheet

DNA recognition by a ß-sheet is discussed in the lightof crystal structures of the MetJ and Arc repressors. The DNAbinding geometry of a ß-sheet can be understood interms of (i) close fitting of the two surfaces and (ii) matchingof residue and base positions. A ß-sheet is not entirelyflat but has a curvature. A ß-sheet of the Met-Arcfamily faces the DNA major groove with its convex surface; thelocal DNA major groove is deepest at the centre. The ß-sheetfollows 6 bp; every two residues face the DNA and the firstand fifth residues, which are separated by 13.2 Å, bind,respectively, to the third and sixth bases, which are separatedby 13.5 Å, on the same DNA strand.