Lipid and fatty acid composition of testes of quaking mice |
| |
Authors: | J G Coniglio W M Grogan Jr D G Harris M L Fitzhugh |
| |
Affiliation: | (1) Department of Biochemistry, Vanderbilt University, 37232 Nashville, Tennessee |
| |
Abstract: | Testes of quaking mice (sterile mutants) and of controls were analyzed for major lipid classes and fatty acid composition.
Of the main lipid classes, only cholesterol esters differed significantly in concentration between the two groups (1.01 for
quakers vs 0.69 mg/g wet wt of tissue for controls). The concentration of triglycerides was 4.5–5.0, that of total phosphatides
18–19, and that of free cholesterol 1.9–2.0 mg/g for mutants and controls. The concentrations of phosphatidyl ethanolamine
and of sphingomyelin were both lower in quaking than in normal mice, but only the change in the former was statistically significant.
Phosphatidyl choline was the major phosphatide (43–45% of total phosphatides) followed by phosphatidyl ethanolamine (24–26%)
and sphingomyelin, phosphatidyl serine, and phosphatidyl inositol (all ca. 7% of total phosphatides). Minor differences between
the mutants and controls were observed in concentrations of fatty acids of major lipid classes. The mutants, sterile because
of faulty spermatid differentiation, had normal quantities of 22∶6 w3 and 22∶5 w6. These data are consistent with the hypothesis
that the 22-carbon polyenes are associated with the formation of spermatids, rather than with their final differentiation
into spermatozoa. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|