酶解法从猪气管中提取硫酸软骨素

以猪气管软骨为原料,利用二次酶解法提取硫酸软骨素,设计正交试验对酶解工艺进行优化,并测定了硫酸软骨素的纯度、含氮量、含氯化物量等主要指标.结果表明:最佳工艺组合条件为温度40℃,酶质量分数1.5％,提取时间2.5h,在此条件下平均得率达到10.98％,纯度达到85.22％.该工艺简便易行,有望为工业生产提供参考.

Enzymatic Preparation of Chondroitin Sulfate from Swine Trachea

In this paper, the preparation of chondroitin sulfate （CS） by two-step enzymatic hydrolysis of swine trachea cartilage was optimized using orthogonal array design. The starting raw material was hydrolyzed sequentially by trypsin and a special-purpose enzyme preparation. The samples obtained were measured for purity and nitrogen and chloride contents. The optimal hydrolysis conditions were determined as 40 ℃, 1.5% of enzyme concentration and 2.5 h. Under these conditions, the average yield of CS was 10.98% and the purity was 85.22%. The optimized process is simple and easy to operate and can provide useful evidence for future industrial production.