固相萃取-HPLC法同时测定肉制品中苋菜红、胭脂红、日落黄和诱惑红的含量

建立了利用固相萃取-高效液相色谱法同时测定肉制品中4种合成色素的检测方法.样品经乙醇、氨水和水溶液(7∶2∶1,mlm)提取后,固相萃取净化,经C18柱分离,用紫外检测器检测.结果表明:4种合成色素分离良好,加标平均回收率为66.7％～99.7％,相对标准偏差1.9％～3.6％.该方法准确度和精密度较高,可应用于测定肉制品中的合成色素.

Simultaneous Determination of Amaranth,Carmine, Sunset Yellow and Allure Red in Meat Products by High Performance Liquid Chromatography with Solid Phase Extraction

This paper studied a method for simultaneous determination of 4 synthetic food colorants including amaranth, carmine, sunset yellow and allure red in meat products by reversed-phase HPLC following solid phase extraction. Samples were extracted with ethanol-aqueous ammonia-water （7：2：1, m/m） and after clean-up by solid phase extraction, the extracts were separated on a C18 cartridge and detected with a UV detector. Good separation was achieved for the four food colorants. The mean spiked recoveries were in the range from 66.7% to 99.7% with RSD between 1.9% and 3.6%. The HPLC method was highly accurate and precise and could be used for the determination of food colorants in meat products.