Microfluidic inverse phase ELISA via manipulation of magnetic beads |
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Authors: | Hong Chen Assem Abolmatty Mohammad Faghri |
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Affiliation: | (1) Department of Mechanical, Industrial, and Systems Engineering, University of Rhode Island, Kingston, RI 02881, USA;(2) Department of Plant Sciences, University of Rhode Island, Kingston, RI 02881, USA;; |
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Abstract: | We report a new technique for conducting immuno-diagnostics on a microfluidic platform. Rather than handling fluid reagents
against a stationary solid phase, the platform manipulates analyte-coated magnetic beads through stationary plugs of fluid
reagents to detect an antigenic analyte. These isolated but accessible plugs are pre-encapsulated in a microchannel by capillary
force. We call this platform microfluidic inverse phase enzyme-linked immunosorbent assay (μIPELISA). μIPELISA has distinctive
advantages in the family of microfluidic immunoassay. In particular, it avoids pumping and valving fluid reagents during assaying,
thus leading to a lab-on-a-chip format that is free of instrumentation for fluid actuation and control. We use μIPELISA to
detect digoxigenin-labeled DNA segments amplified from E. coli O157:H7 by polymerase chain reaction (PCR), and compare its detection capability with that of microplate ELISA. For 0.259 ng μl−1 of digoxigenin-labeled amplicon, μIPELISA is as responsive as the microplate ELISA. Also, we simultaneously conduct μIPELISA
in two parallel microchannels. |
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