不同产地马铃薯果肉与皮中总酚和单体酚类物质的测定与比较

目的：运用高效液相色谱法和福林酚比色法对新疆、江西、福建和北京马铃薯果肉与皮中的单体多酚和总酚含量进行测定与比较。方法：色谱柱YMC-C18柱(250mm×4.6mm，5μm)，流动相：甲醇(A)-0.5%磷酸溶液(B)梯度洗脱。结果：绿原酸、咖啡酸、对香豆酸、阿魏酸、芦丁均能达到基线分离，各成分都有较宽的线性范围和良好的线性关系，加样回收率在91.36%～102.23%。总酚含量为0.238～1.190mg/g，其中果肉中绿原酸占果肉总酚含量的80.59%～89.06%；皮中绿原酸占皮中总酚含量的65.54%～80.00%。结论：在此色谱条件下各种单体酚类物质均得到良好的分离，该方法快速、准确、可靠、重复性好。同时福林酚比色法适合于马铃薯总酚含量的测定。

Determination of Total and Individual Phenolics in Potato Flesh and Skin from Different Growing Areas

The contents of total and individual phenolics in potato flesh and skin from different growing areas in Xinjiang,Jiangxi,Fujian and Beijing were comparatively determined by both HPLC and Folin-Ciocalteu colorimetry.The gradient elution of phenolics was carried out on YMC-C18 column(250 mm × 4.6 mm,5 μm) using methanol(mobile phase A) and.5% phosphoric acid(mobile phase B).Baseline separation was observed for chlorogenic acid,caffeic acid,p-coumaric acid,ferulic acid and rutin.All of them showed good linear relationship over a wide concentration range.The average spike recoveries for them from a known sample were 91.36%-102.23%(n = 3).The total phenolic contents in all samples under investigation were 0.238-1.190 mg/g.Chlorogenic acid accounted for 80.59%-89.06% of the total phenolics in potato flesh compared to 65.54%-80.00% in potato skin.The HPLC method was quick,accurate,reliable and repeatable.The Folin-Ciocalteu colorimetry method was more suitable for the determination of total phenolics in potato.