| 溶菌酶活力测定方法的改进 |
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| 引用本文： | 谢红伟.溶菌酶活力测定方法的改进[J].食品科学,2003,24(9):119-121. |
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| 作者姓名： | 谢红伟 |
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| 作者单位： | 贵州大学 |
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| 摘 要： | 本文以壳聚糖代替溶壁微球菌作底物测定溶菌酶的活力。实验证明:在55℃,pH=4.5时,达到最大酶反应速度。酶浓度在0～4.5U/ml范围内符合郎伯-比尔定律,回归方程为Y=0.2173X-0.0114,线性相关系数为γ=0.9998。
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| 关 键 词： | 溶菌酶 壳聚糖 酶活力 溶壁微球菌 |
| 文章编号： | 1002-6630(2003)09-0122-03 |
Improvement of Lysozyme Activity Measurement |
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| Xie Hongwei.Improvement of Lysozyme Activity Measurement[J].Food Science,2003,24(9):119-121. |
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| Authors: | Xie Hongwei |
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| Affiliation: | Xie Hongwei |
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| Abstract: | Chitosan was used to replace the substrate M.Lysodeikticus to determine the enzyme activity of lysozyme.It showedthat the optimum temperature and optimum pH were 55℃ and 4.5 respectively and it was accordant with the lamber-bier lawwhen the enzyme concentration was 0～4.5U/ml.The regression equation is Y=0.2173X-0.0114 with linear correlationcoefficient as 0.9989. |
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| Keywords: | chitosan lysozyme enzyme activity M Lysodeikticus |
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