高产SOD酵母菌的诱变选育及发酵条件研究

用紫外线诱变酿酒酵母B094原生质体，得到1株SOD含量较高的菌株BUV094，其SOD含量为720U／g湿菌体。用正交试验及单因了条件试验对该突变株进行培养基组成及发酵条件的研究，结果表明：其最佳培养基组成为葡萄糖2％，蛋白胨1％，酵母膏1％，7Be麦芽汁9％（V／V），CuSO475mmol／ml，ZnSO425mmol／ml；最佳培养条件为：初始pH5．5，摇瓶装量为75ml／500ml三角瓶，接种量为15％，培养时间24h。在此条件下，该菌细胞生物量为5．39g／100ml，SOD含量为1024U/g湿菌体，SOD产量为5520U／100ml，SOD产量较出发菌株提高103％。

Study on Breeding and Fermentation Conditions of High-yield SOD from Sacdharomyces cerevisiace

A SOD high-yield mutant was filtrated out through UV treatment to protolast of Sacdharomyces cerevisiace. The SOD activity was 720U/g fresh cell. The medium and fermentation condition was studied. It showed that the optimal medium was consisted of 2% glucose, 9%(V/V) malt wort, 1%peptone, 1%yeast extract, CuSO4 75mmol/ml ZnSO4 25mmol/ml. The optimal culture conditions were as follows:initial pH 5.5, medium cubage 75ml/500ml, 15%concentration inoculated and culture time 24h. In this condition,the biomass was 5.39g/100ml, SOD activity was 1024U/g fresh cell and SOD production was 5520U/ 100ml, yield 103% times of original strain.